ABSTRACT

In our study, the peptide synthesis catalyzed by trypsin between four different amino acids (prolin, tyrosin, histidin and tryptophan) and glicine were performed in biphasic and homogen systems. It was determined that effect of iso octane and bensen, ethylmethyl ketone, iso amylalcohol, iso propanol as reaction medium and solvent on peptide synthesis. Moreover, In order to determine effect of water content (5, 10, 15, 20%) in the reaction medium, peptide synthesis were carried out in ethylmethyl ketone and iso amylalcohol, iso propanol. The best of peptide formation obtained with ethylmethyl ketone which consist of 10-15% buffer. In presence of bensen and iso octane, the expected peptide products were not obtained but peptides were synthesized with iso amylalcohol and iso propanol. The product analysis were carried out by using the paper and thin-layer chromatography.