Abstract

Rosmarinic acid was isolated from ethanol extractions obtained from Prunella grandiflora L. and P. vulgaris L. The total phenolic content of ethanol fractions during isolation was determined by the Folin-Ciocateu method. The cytotoxic doses of the isolated rosmarinic acid were determined by WST-1 (Roche Applied Sciences, Mannheim, Germany) cell proliferation assay. A 10-60ng cytotoxic dose was determined for pancreas (PANC-1), prostate (PC-3), colon (HT-29) and breast (MDAMB 436) cancers and GBM (T98G) cell lines and lymphatic tissues. 24 and 48h incubation periods were applied during dose determinations. An antiproliferative effect was observed at the end of 48h incubation period with 50ng rosmaniric acid treatment in PC3 cell line and with 60ng treatment in PANC-1, HT-29, MDA-MB 436 and T98G cell lines. No cytotoxic activity of rosmarinic acid was observed in non-tumor cells.