ABSTRACT

In this study the cytogenetic effects of Maleic hydrazide (MH) at different concentration and treatment periods were investigated in peripheral blood culture. For this purpose, 75, 300, 500, 1000 µg/ml concentrations of MH dissolved in distilled water were used. Human lymphocytes were treated with MH at different concentrations for 24 and 48 hours. Chromosome abnormalities were observed such as chromatid gap, chromatid break, isochromatid gap and isochromatid break. Results were evaluated in two ways. If gaps are accepted as chromosome aberrations (CA), at all the groups MH significantly induced CA except 75 µg/ml 48 hour treatment group compared with control. If not, it was observed that MH is not affective. MH also decreased mitotic index (MI) depending upon the increasing concentrations and treatment periods.